Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2

Simple item page
dc.contributor.authorRohaim, M.A.
dc.contributor.authorClayton, E.
dc.contributor.authorSahin, I.
dc.contributor.authorJulianne, V.
dc.contributor.authorKhalifa, M.E.
dc.contributor.authorAl-Natour, M.Q.
dc.contributor.authorBayoumi, M.
dc.contributor.authorPoirier, M.C.
dc.contributor.authorBranavan, M.
dc.contributor.authorMukunthan, T.
dc.contributor.authorChaudhry, N.S.
dc.contributor.authorRavinder, S.
dc.contributor.authorAmy, B.
dc.contributor.authorPeter, B.
dc.contributor.authorHacking, W.
dc.contributor.authorBotham, J.
dc.contributor.authorBoyce, J.
dc.contributor.authorWilkinson, H.
dc.contributor.authorWilliams, C.
dc.contributor.authorWhittingham-Dowd, J.
dc.contributor.authorShaw, E.
dc.contributor.authorMatt Hodges
dc.contributor.authorLisa Butler
dc.contributor.authorBates, M.D.
dc.contributor.authorRoberto La, R.
dc.contributor.authorBalachandran, V.
dc.contributor.authorAnil, F.
dc.contributor.authorMunir, M.
dc.date.accessioned2023-12-29T08:15:35Z
dc.date.available2023-12-29T08:15:35Z
dc.date.issued2020
dc.description.abstractUntilvaccinesandeffectivetherapeuticsbecomeavailable,thepracticalsolutiontotransit safelyoutofthecurrentcoronavirusdisease19(CoVID-19)lockdownmayincludetheimplementation ofaneffectivetesting,tracingandtrackingsystem.However,thisrequiresareliableandclinically validateddiagnosticplatformforthesensitiveandspecificidentificationofSARS-CoV-2.Here, wereportonthedevelopmentofadenovo,high-resolutionandcomparativegenomicsguided reverse-transcribedloop-mediatedisothermalamplification(LAMP)assay.Tofurtherenhancethe assayperformanceandtoremoveanysubjectivityassociatedwithoperatorinterpretationofresults, weengineeredanovelhand-heldsmartdiagnosticdevice.Therobustdiagnosticdevicewasfurther furnishedwithautomatedimageacquisitionandprocessingalgorithmsandthecollateddatawas processedthroughartificialintelligence(AI)pipelinestofurtherreducetheassayruntimeandthe subjectivityofthecolorimetricLAMPdetection.ThisadvancedAIalgorithm-implementedLAMP (ai-LAMP)assay,targetingtheRNA-dependentRNApolymerasegene,showedhighanalytical sensitivityandspecificityforSARS-CoV-2.Atotalof~200coronavirusdisease(CoVID-19)-suspected NHSpatientsamplesweretestedusingtheplatformanditwasshowntobereliable,highlyspecific andsignificantlymoresensitivethanthecurrentgoldstandardqRT-PCR.Therefore,thissystemcould provideanefficientandcost-effectiveplatformtodetectSARS-CoV-2inresource-limitedlaboratories.en_US
dc.identifier.doidoi:10.3390/v12090972en_US
dc.identifier.urihttp://repo.lib.jfn.ac.lk/ujrr/handle/123456789/10023
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectSARS-CoV-2en_US
dc.subjectDiagnosisen_US
dc.subjectLAMPen_US
dc.subjectPoint of careen_US
dc.subjectArtificial intelligenceen_US
dc.titleArtificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2en_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid Detection of SARS-CoV-2_viruses-12-00972-v2.pdf
Size:
3.83 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Electrical & Electronic Engineering